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Departments: LC/MS/MS Can Help Screen Cereal
Water-soluble vitamins, which are very polar, have poor retention on reverse phase columns. The presence of ion pair reagents such as heptafluorobutyric acid in the mobile phase has been shown to improve the separation and retention of these compounds. The drawback of using such ion pair reagents, however, is the high background levels generated inside the mass spectrometer (MS). Therefore, we have developed a rapid and sensitive method with ammonium formate in the mobile phase solvent using a column with...